Analysis Note

single band by SDS-PAGE

Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

General description

Single chain glycoprotein present in normal serum at about 23 mg/100 ml. Principal physiological inhibitor of thrombin. Present at higher levels in plasma than in serum because it complexes with thrombin during coagulation. Functions by inhibiting proteolytic enzymes involved in blood coagulation and fibrinolysis, including Factor Xa, plasmin, thrombin, and trypsin. Potency is strongly enhanced by heparin.

Single-chain glycoprotein present in normal serum at about 23 mg/100 ml. Principal physiological inhibitor of thrombin. Present at higher levels in plasma than in serum because it complexes with thrombin during coagulation. Functions by inhibiting proteolytic enzymes involved in blood coagulation and fibrinolysis, including Factor Xa, plasmin, thrombin, and trypsin. Potency is strongly enhanced by heparin.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Tsiang, M., et al. 1997. J. Biol. Chem. 272, 12024.

Packaging

1 mg in Glass bottle

Physical form

Lyophilized from 100 mM sodium citrate buffer, 20 mM Tris-HCl, pH 8.3.

Preparation Note

Please refer to label for lot-specific volume prior to lyophilization.

Unit Definition

One unit is defined as the amount of antithrombin that will inhibit 1.0 unit of human plasma thrombin present in excess. Thrombin activity is measured using 500 µM tosyl-Gly-Pro-Arg-pNA as substrate in 100 mM Tris-HCl at 25°C, pH 8.2

Warning

Toxicity: Standard Handling (A)